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Digital PCR (sometimes called digital or clonal amplification) of many single nucleic acids to form large clonal populations of amplicons is an essential step in 2nd generation sequencing methods by 454, Illumina, Polonator and Applied Biosystems. While the technology and platforms vary, it is digital PCR that enables the cost and time of sequencing individual genomes to be reduced by orders of magnitude. |
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Digital PCR amplification of nucleic acid fragments in emulsions (with and without beads) is one of many methods for producing detectable concentrations of clonal product for sequencing. Digital PCR amplification of single, isolated nucleic acid fragments restrained on small surface regions produces detectable concentrations of clonal amplicons. Digital PCR amplification of DNA fragments in micro emulsions is a key step in SOLID technology. |
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